High-selectivity cytology via lab-on-a-disc western blotting of individual cells
نویسندگان
چکیده
منابع مشابه
Subcellular western blotting of single cells
Although immunoassays are the de facto standard for determining subcellular protein localization in individual cells, antibody probe cross-reactivity and fixation artifacts remain confounding factors. To enhance selectivity while providing single-cell resolution, we introduce a subcellular western blotting technique capable of separately assaying proteins in the 14 pL cytoplasm and 2 pL nucleus...
متن کاملWestern Blotting
Histological Analysis and Immunohistochemistry Renal sections (3 μm thick) were stained with periodic acid-Schiff (PAS) and examined under light microscopy. Glomerulosclerosis index was semiquantitatively graded as 0 to 4+; vascular injury score as 0 to 3+; and tubulointerstitial injury score as 0 to 5+, according to the criteria reported previously. Ultrastructure of glomerular podocytes was a...
متن کاملMicrofluidic Western blotting.
Rapid, quantitative Western blotting is a long-sought bioanalytical goal in the life sciences. To this end, we describe a Western blotting assay conducted in a single glass microchannel under purely electronic control. The μWestern blot is comprised of multiple steps: sample enrichment, protein sizing, protein immobilization (blotting), and in situ antibody probing. To validate the microfluidic...
متن کاملImmunoprecipitation and western blotting
SKG or BALB/c T cells (3.0 £ 10) were stimulated for 72 h with plate-bound anti-CD3 mAb (2C11) in the presence or absence of plate-bound anti-CD28 mAb in RPMI-1640 medium supplemented with 10% fetal calf serum and 50 mM 2-mercaptoethanol. Cells were also stimulated with TPA (1.4 ng ml) and ionomycin (0.14 mM). KJ1.26þ T cells from DO or DO.SKG mice were stimulated with ovalbumin (323–339) pepti...
متن کاملMiniaturized Lab-on-a-Disc (miniLOAD).
A miniaturized centrifugal microfluidic platform for lab-on-a-chip applications is presented. Unlike its macroscopic Lab-on-a-CD counterpart, the miniature Lab-on-a-Disc (miniLOAD) device does not require moving parts to drive rotation of the disc, is inexpensive, disposable, and significantly smaller, comprising a 10-mm-diameter SU-8 disc fabricated through two-step photolithography. The disc ...
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ژورنال
عنوان ژورنال: Lab on a Chip
سال: 2017
ISSN: 1473-0197,1473-0189
DOI: 10.1039/c6lc01333c